Acupuncture
Aleksey Radchenko, Certified specialist in acupuncture
Cigun
Homeopathy
Svetlana Laputjko, Specialist in homeopathy
Psychotherapy
Psychotherapist Alexander Moshkin
Atmest smēķēšanu
Caurdurt ausis
  about us specialists price-list contact us   lv ru en  
DiagnosticsTreatmentBemer-Therapy


ROS ROS
                           


    News of medicine.
 
 

Alternative splicing is crucial to muscle mass

 
 

12/10/2018

Despite the importance that changes in muscle mass have in aging, overall body metabolism and in chronic disease, we still don't fully understand the mechanisms that contribute to the maintenance of adult muscle mass.

"A number of cell signaling and metabolic pathways have been studied regarding their involvement in sustaining adult muscle mass, but not alternative splicing," said Dr. Thomas Cooper, professor of pathology & immunology, of molecular and cellular biology and of molecular physiology and biophysics at Baylor College of Medicine.

Alternative splicing is a cellular mechanism that allows cells to produce many different proteins from a single gene. A gene can be visualized as a short string of 'beads' or exons. Each bead codes for a different piece of the final protein. Alternative splicing allows the cell to make different proteins by combining the 'beads' in different ways.

The researchers and other groups previously determined that alternative splicing regulators Rbfox1 and Rbfox2 were required for muscle development and function, but Cooper's group hypothesized that the two proteins worked together, and their combined role in adult muscle had not been studied before.

"To determine whether alternative splicing played a role in adult muscle maintenance, we disrupted the process in adult mice by knocking out the genes Rbfox1 and Rbfox2 only in skeletal muscles. Then, we looked at the effect this disruption had in muscles in the animals' limbs," said Dr. Ravi Singh, instructor of pathology & immunology at Baylor College of Medicine.

The critical role of alternative splicing

"We observed that the knockout mice rapidly lost about half of their skeletal muscle mass within four weeks," Singh said. "Two weeks after knocking out the genes Rbfox1 and Rbfox2, hundreds of other genes altered their expression and other genes their alternative splicing, including the capn3 gene, which switched splicing to produce an active form of a protease, an enzyme that degrades proteins."

"Taking all our observations together, we attributed the loss of muscle mass to an increase in degradation of muscle protein rather than a reduction of protein synthesis," said Cooper, who also is the S. Donald Greenberg and R. Clarence and Irene H. Fulbright Professor and a member of the Dan L Duncan Comprehensive Cancer Center at Baylor.

"The results indicate that the Rbfox splicing regulators, which are highly conserved from the worm C. elegans to humans, are essential for maintaining skeletal muscle mass in adult mice." "Our contribution has implications for studies on the role of muscle mass in metabolism and on muscle mass loss in aging and chronic disease," Singh said.

Read all the details of this study in the journal Cell Reports.

 
   
 

sciencedaily.com

 
 
 
 
 

Svetlana Laputko    trauma or stroke    neuroses    Aleksey Radchenko    psychiatrist    Psychoanalysis    Igor Shelegov    children and adolescents    teenagers    Acupuncture    Acupuncture   intervertebral disks    intervertebral disks   Alexander Moshkin    neuroses    trauma or stroke    sonography    neuroses    ultrasonography    Svetlana Laputko    Aleksey Radchenko   Acupuncture    Psychoanalysis    psychiatrist    children and adolescents    intervertebral disks    teenagers    Igor Shelegov    Acupuncture    neuroses    Acupuncture    sonography   Alexander Moshkin   intervertebral disks    Svetlana Laputko    ultrasonography   Acupuncture    trauma or stroke    Aleksey Radchenko    psychiatrist    intervertebral disks    neuroses    Psychoanalysis    children and adolescents    Igor Shelegov    Acupuncture   Alexander Moshkin    Svetlana Laputko    Aleksey Radchenko    teenagers    Acupuncture    neuroses    Igor Shelegov    sonography   intervertebral disks    ultrasonography    trauma or stroke    Acupuncture   Alexander Moshkin    neuroses    Psychoanalysis    children and adolescents    teenagers   Acupuncture    psychiatrist    Acupuncture    intervertebral disks    Svetlana Laputko    neuroses   intervertebral disks   

All rights are protected. © 'ROS' Ltd, 2008
PULS.LV Professional statistical system       Europuls.eu - Catalog of the European Internet resources      Яндекс.Метрика Auraj studio карта сайта